FastQCFastQC Report
Wed 30 Nov 2022
4061-JW32trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW32trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142001
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG6801047.894028915289326No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC32262.2718149872184No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC29362.0675910733023004No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG25891.823226596995796No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG18421.297173963563637No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA12840.9042189843733496No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG11760.8281631819494228No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC9320.6563334061027739No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG9020.6352067943183498No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA8980.6323899127470933No Hit
TCAAGAACATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC7970.5612636530728657No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG7500.5281652946106014No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA7160.5042218012549208No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG6750.4753487651495412No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG6540.46056013690044434No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA5680.3999971831184288No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC5600.3943634199759157No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA5280.37182836740586334No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA5060.35633551876395236No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT4740.3338004661939No Hit
CAAGGAATTTTCCCCAATGGCCGAAAGGCTGAGGGAGCAACGCCGCGTGG4550.3204202787304315No Hit
GCGCGAAAACTTTACACTGCGGGAAACCGCGATAAGGGGACCCCGAGTGC4500.3168991767663608No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA4340.3056316504813346No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG4300.3028147689100781No Hit
CAAGGAATTTTCCCCAATGGCCTAACGGCTGAGGGAGCAACGCCGCGTGG3660.25774466376997346No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG3530.2485897986633897No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA3480.245068696699319No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG3440.2422518151280625No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC3130.2204209829508243No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA3120.21971676255801015No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA3050.21478721980831122No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA2950.20774501588016986No Hit
TGAGGAATATTGCTCAATGGGCGAAAGCCTGAAGCAGCAACGCCGCGTGC2920.20563235470172744No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA2890.20351969352328503No Hit
TGAGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2870.20211125273765676No Hit
TCGAGAAGCTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGA2800.19718170998795784No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG2740.192956387631073No Hit
TAGGGAATATTGGTTAATGGACGAAAGTCTGAACCAGCAACGCCGCGTGT2700.1901395060598165No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2690.18943528566700235No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG2570.18098464095323272No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT2540.1788719797747903No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG2510.17675931859634791No Hit
ACGCTTTCGTCGATAACATAGAGGTCGTCGCCCGGAGTGATGGCCGTAAC2470.17394243702509138No Hit
TAGGGAATATTGCATAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT2460.17323821663227723No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGG2420.1704213350610207No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG2370.16690023309695No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG2320.16337913113287933No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA2270.15985802916880867No Hit
TGAGGAATATTGCGCAAGGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG2250.1584495883831804No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC2240.15774536799036626No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC2230.1570411475975521No Hit
TAGGGAATATTGCTCAATGGGCGAAAGCCTGAAGCAGCAACGCCGCGTGC2080.14647784170534012No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA2050.1443651805268977No Hit
GCGCGAAAACTTTACAATGCCGGCAACGGCGATAAGGGGACCTCGAGTGC2030.1429567397412694No Hit
CTAAGAATCTTCCGCAATGGGGGAAACCCTGACGGAGCGACGCCGCGTGG1990.1401398581700129No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1980.13943563777719875No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA1950.13732297659875634No Hit
TGAGGAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGG1920.13521031542031395No Hit
AGGATAGTGGAATTCCCGGTGTAGTGGTGAAATGCGTAGATATCGGGAGG1920.13521031542031395No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG1880.1323934338490574No Hit
TGAGGAATCTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1820.1281681114921726No Hit
TGAGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1780.12535122992091605No Hit
CAAGGAATTTTCCCCAATGGACGCAAGTCTGAGGGAGCAACGCCGCGTGG1750.12323856874247364No Hit
CAAGGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGG1710.12042168717121711No Hit
TGAGGAATATTGCGCAATGGGCGCAAGCCTGACGCAGCAACGCCGCGTGG1710.12042168717121711No Hit
TGAGGGATTTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGA1700.11971746677840298No Hit
TGAGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1670.11760480559996056No Hit
GAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGA1650.11619636481433229No Hit
TCGAGAGGCTTCGGCAATGGGGGAAACCCTGACCGAGCGACGCCGCGTGG1630.11478792402870401No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC1500.10563305892212027No Hit
TTCAGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGATGCCGCGTGA1490.10492883852930614No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA1470.10352039774367786No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG1460.10281617735086372No Hit
TAGGGAATATTGCACAATGGGCGAATGCCTGATGCAGCAACGCCGCGTGC1460.10281617735086372No Hit
TCGAGAATTTTTCACAATGGGCGCAAGCCTGATGGAGCGACGCCGCGTGG1440.10140773656523545No Hit
TCGAGAATTTTTCACAATGGGCGAAAGCCTGATGGAGCGACGCCGCGTGG1440.10140773656523545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTG450.0228.000026
CCTTCGC100.0018291178228.09
ACTATTC100.0018291178228.06
TAGAGGC152.0205627E-5228.03
ATTAGTA100.0018291178228.09
CTTCCAC302.7284841E-11228.09
CAACGAA100.0018291178228.01
TCTTCCA202.2284257E-7228.08
GATTTTG100.0018291178228.06
TATATTG152.0205627E-5228.06
TTTCCAC100.0018291178228.09
TATGCGC100.0018291178228.09
AATATTA400.0228.06
CTTGGAC252.4701876E-9228.09
TATTGAG350.0228.08
ATTCCCC152.0205627E-5228.09
GCTTCCA100.0018291178228.08
AACATTG152.0205627E-5228.06
TATTCCT152.0205627E-5228.08
TATTCCC152.0205627E-5228.08
Produced by FastQC (version 0.11.9)