FastQCFastQC Report
Wed 30 Nov 2022
4061-JW38trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW38trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length234
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA87.6190476190476195No Hit
CCCTAGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA43.8095238095238098No Hit
CCCGGGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA43.8095238095238098No Hit
CCCCAGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA43.8095238095238098No Hit
CCCCGGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA43.8095238095238098No Hit
CCCTCGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA32.857142857142857No Hit
CCCGCGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA32.857142857142857No Hit
GGATTATTTAGAAACCCTGGTAGTCCTGTCTCTTATACACATCTCCGAGC32.857142857142857No Hit
CCCCCGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA32.857142857142857No Hit
CCCCTGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA32.857142857142857No Hit
CCCTTGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA21.9047619047619049No Hit
GGAGAGATTGTCACGCGGATTTCTGGCAGTTAGAAACCCTAGTAGTCCTG21.9047619047619049No Hit
AAACCCCAGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG21.9047619047619049No Hit
GGATTATTAGAAACCCGCGTAGTCCTGTCTCTTATACACATCTCCGAGCC21.9047619047619049No Hit
CCCGTGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAA21.9047619047619049No Hit
CGGCAGCGTCAGATGTGTATTAGAAACCCGGGTAGTCCTGTCTCTTATAC21.9047619047619049No Hit
ACAAATTTAAATTTCCCTGAGATTTTTTAGCTCCTGTTAGAAACCCGAGT21.9047619047619049No Hit
GGATTATTTAGAAACCCGTGTAGTCCTGTCTCTTATACACATCTCCGAGC21.9047619047619049No Hit
GGATTATTTAGAAACCCGCGTAGTCCTGTCTCTTATACACATCTCCGAGC10.9523809523809524No Hit
GGATTATTTAGAAACCCCAGTAGTCCTGTCTCTTATACACATCTCCGAGC10.9523809523809524No Hit
TGCGGAGTCCTGTGTCTTATACACATCTCCGACGCCACAAGACAGTAAAG10.9523809523809524No Hit
TGATAGATGTTGGCCAAGGGGCCTAGGCCTGAAGCGTCTCAACCGCTCGC10.9523809523809524No Hit
TCGGGGATGTTGAGGAAGGGACGTAGGTGTGAGTCAGCTAGACCGCGGGC10.9523809523809524No Hit
TTAGAAACCCTGGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAG10.9523809523809524No Hit
GAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA10.9523809523809524No Hit
TCGAGGAAGCTTAACAATACTCGCAGCCGTTATTCGTAGAGATCAGGTGC10.9523809523809524No Hit
CCCTCGTAGTCCTGTCTCTTATACACATCGCCGTGCGCACGAGACAGTAA10.9523809523809524No Hit
GGATTATTAGATCCCCGTGTAGTCCTGTCTCTTATACACATCTCCGAGCC10.9523809523809524No Hit
AGAAACCCTCGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10.9523809523809524No Hit
CCCGAGTAGTCCCGTCTCTTATACGCATCTCCGAGCCCACGAGACAGTAA10.9523809523809524No Hit
CTAGATACCCGGGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAG10.9523809523809524No Hit
CTAGAAACCCCCGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAG10.9523809523809524No Hit
GGATTATAGAAACCCTAGTAGTCCTGTCTCTTATACCACATCTCCGAGCC10.9523809523809524No Hit
TGTAGAAACTCGCGCAATCCTGTCTCTTCTGACCCTGCCCCGCCCCGCGA10.9523809523809524No Hit
GGATTATTTAGAAACCCCCGTAGTCCTGTCTCTTATACACATCTCCGAGC10.9523809523809524No Hit
AATAGATACCCGCGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGA10.9523809523809524No Hit
TTAGATACCCCAGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAG10.9523809523809524No Hit
CCCGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAAAC10.9523809523809524No Hit
GGATTATAGAAACCCCGGTAGTCCTGTCTCTTATACACATCTCCGAGCCC10.9523809523809524No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTTTAGAAACCCTTGTAGTCCTG10.9523809523809524Illumina Single End PCR Primer 1 (97% over 34bp)
CTAGAAACCCTGGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAG10.9523809523809524No Hit
GGATTATTAGAAACCCGCGTAGGCCTGTCTCTTATACACATCTCCGAGCC10.9523809523809524No Hit
GGATTATTAGAAACCCTGGTAGTCCTGTCTCTTATACACATCTCCGAGCC10.9523809523809524No Hit
CCCGGGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTAC10.9523809523809524No Hit
AGATACCCGTGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10.9523809523809524No Hit
CGTCGGCAGCGTCAGATGTGTATTAGAAACCCCAGTAGTCCTGTCTCTTA10.9523809523809524No Hit
AAATATGTGTGGGTTGAGGTGTAATTAGAAACCCCAGTAGTCCTGTCTCT10.9523809523809524No Hit
GGATTATTAGATACCCTAGTAGTCCTGTCTCTTATACACATCTCCGAGCC10.9523809523809524No Hit
CCCCCGTAGTCCTGTCTCTTATACACATCTCCGGGCCCACGAGACAGTAA10.9523809523809524No Hit
ACCCTCGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTA10.9523809523809524No Hit
TCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTAAACAATATCG10.9523809523809524No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTTTAGAAACCCGCGTAGTCCTG10.9523809523809524Illumina Single End PCR Primer 1 (97% over 34bp)
GGAGAGATTGTCACGCGGATTTCTGGCAGTTAGATACCCCCGTAGTCCTG10.9523809523809524No Hit
ATCGGAAGAGCGTCGTGGAGGTAAAGATTTTAGAAACCCTCGTGGTCCTG10.9523809523809524Illumina Single End PCR Primer 1 (95% over 21bp)
CCCTAGTAGTCCTGTCTCTTAATACACATCTCCGAGCCCACGAGACAGTA10.9523809523809524No Hit
GGAGTATTTAGAAACCCTGGTAGTCCTGCCTCTTATACACATCTCCGATC10.9523809523809524No Hit
TGAGGAATGTTGACCAATGGCGGAAGGCGTGAGGCAGCAAGGCCGCGTGG10.9523809523809524No Hit
TGAGGGATATAGCGGATGGATCGAAGACGTGAGGCAGAAACACCGGGTGG10.9523809523809524No Hit
AGTAGAAACCCGCGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGA10.9523809523809524No Hit
GGAGTCATGTCTCATATACAGATCTCCGCGCCTACGCGACAGTCGCCAGT10.9523809523809524No Hit
AAACCCGAGTAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG10.9523809523809524No Hit
TGCGGGATGTTGCGGAAGGGGAGTAGGTGTAAGGGGGCTAGACCGCGCGG10.9523809523809524No Hit
ATCGGAAGAGCGTCGTGTAGGGGACGAGTTTAGAAACTCTTGTAGTCCTG10.9523809523809524Illumina Single End PCR Primer 1 (95% over 24bp)
GGATTATTTAGATACCCGCGTAGTCCTGTCTCTTATACACATCTCCGAGC10.9523809523809524No Hit
TCAGGGACGTTGCCCAAGGGTCCAAGATCTGACGCATCTTCATCGCTTGC10.9523809523809524No Hit
TTAGATACCCCAGCAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAG10.9523809523809524No Hit
GGATTATGAAACCCGAGTAGTCCTGTCTCTTATACACATCTCCGAGCCCA10.9523809523809524No Hit
GCCCTGTTGTCCAATCTCGGATACACTTCTGCCGCCCCACGACGCGGGAA10.9523809523809524No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA451.8996844E-425.33333480-84
Produced by FastQC (version 0.11.9)